This product has the following functions.
Phosphate buffer is a mobile phase that has been commonly used in LC that use detectors such as UV.
The reason is that it has a low background because it does not absorb UV light, and it has a buffering capacity in a wide wavelength range.
However, since phosphate is non-volatile, there is a problem that it precipitates at the ion source of MS ESI or APCI and causes clogging of capillaries or it contaminates the ion source and raises the background for a long time. Furthermore, since phosphate is easily ionized, there is a big problem that it suppresses the ionization of the compound to be analyzed.
Since Salinac Tube adsorbs phosphate, it solves the problems above and enables LC/MS measurement using phosphate buffer.
TFA (trifluoroacetic acid) is often used as a mobile phase when analyzing basic compounds with LC in peptides and small molecules.
Since it is volatile, it can be used for LC/MS, but it is extremely acidic and causes ionization suppression of the analysis target.
By removing TFA with a Salinac tube, LC/MS measurement by TFA is possible.
During ESI ionization, the addition of trace amounts of Na+ and K+ in the solvent to the object to be analyzed complicates the mass spectrum.
Therefore, it is required to obtain a simple and easy-to-analyze mass spectrum of only H+ adduct ions without Na+ or K+ adduct ions.
Salinac tube achieves this by adsorbing Na+ and K+ ions.
In addition, the ions generated by the addition of Na+ and K+ are structurally stable and difficult to cleave with the CID of MS/MS, making MS/MS measurement difficult. However, by using Salinac tube, it can be aggregated into H+ adduct ions and MS/MS measurement turns to be possible.
However, if Na+ or K+ is more likely to be added than H+ due to the nature of the original target compound, the effect of Salinac Tube will not be exhibited, so please consult us in advance.
|CFAN||Online LC/MS measurement of phosphate buffer|
|OOAN||Decreasing added ions, e.g. Na and K|
|CFOO||Online LC/MS measurement of phosphoric acid, ammonium phosphate eluent|
|Usable eluent||Acetonitrile, methanol, water|
|Usable pH||2 - 12|
|Non-measurable compound|| CFAN : Strong ionic compound with phosphate group, sulfonic acid group, quaternary ammonium group, etc. |
OOAN : A strongly basic compound with a quaternary ammonium group, etc.
CFOO : A Strongly acid compound with phosphoric acid group, sulfonic acid group, etc.
With phosphate buffer, LC/MS measurement is along the time.
Na adduct ions decrease.
1. Trifluoroacetic acid eluent concentration become 1/100.
2. Even TFA eluent even detect negative ions.