Salinac Tube

This product has the following functions.

1. LC/MS measurement by using phosphate buffer as a mobile phase.

Phosphate buffer is a mobile phase that has been commonly used in LC that use detectors such as UV.
The reason is that it has a low background because it does not absorb UV light, and it has a buffering capacity in a wide wavelength range.
However, since phosphate is non-volatile, there is a problem that it precipitates at the ion source of MS ESI or APCI and causes clogging of capillaries or it contaminates the ion source and raises the background for a long time. Furthermore, since phosphate is easily ionized, there is a big problem that it suppresses the ionization of the compound to be analyzed.
Since Salinac Tube adsorbs phosphate, it solves the problems above and enables LC/MS measurement using phosphate buffer.

2. LC/MS measurement by using TFA as a mobile phase.

TFA (trifluoroacetic acid) is often used as a mobile phase when analyzing basic compounds with LC in peptides and small molecules.
Since it is volatile, it can be used for LC/MS, but it is extremely acidic and causes ionization suppression of the analysis target.
By removing TFA with a Salinac tube, LC/MS measurement by TFA is possible.

3. ESI ionization when Na+ and K+ are contained in the solution.

During ESI ionization, the addition of trace amounts of Na+ and K+ in the solvent to the object to be analyzed complicates the mass spectrum.
Therefore, it is required to obtain a simple and easy-to-analyze mass spectrum of only H+ adduct ions without Na+ or K+ adduct ions.
Salinac tube achieves this by adsorbing Na+ and K+ ions.
In addition, the ions generated by the addition of Na+ and K+ are structurally stable and difficult to cleave with the CID of MS/MS, making MS/MS measurement difficult. However, by using Salinac tube, it can be aggregated into H+ adduct ions and MS/MS measurement turns to be possible.
However, if Na+ or K+ is more likely to be added than H+ due to the nature of the original target compound, the effect of Salinac Tube will not be exhibited, so please consult us in advance.

Connection diagram

Product image

- Compatible for flow rate 0.2~1.0 mL/min
- Disposable type
- Easy operation (just connect between UV detector and MS)
[Patent pended]

Salinac Tube has three types.

CFANOnline LC/MS measurement of phosphate buffer
OOANDecreasing added ions, e.g. Na and K
CFOOOnline LC/MS measurement of phosphoric acid, ammonium phosphate eluent

Usable eluentAcetonitrile, methanol, water
Usable pH2 - 12
Non-measurable compound CFAN : Strong ionic compound with phosphate group, sulfonic acid group, quaternary ammonium group, etc.
OOAN : A strongly basic compound with a quaternary ammonium group, etc.
CFOO : A Strongly acid compound with phosphoric acid group, sulfonic acid group, etc.

CFAN : LC/MS measurement with phosphate buffer

With phosphate buffer, LC/MS measurement is along the time.

OOAN : Removal of Na adduct ions

Na adduct ions decrease.

CFOO : LC/MS measurement of negative ion with trifluoroacetic acid eluent

1. Trifluoroacetic acid eluent concentration become 1/100.
2. Even TFA eluent even detect negative ions.

IMS (Imaging Mass Spectrometry)

IMS is a technique to visualize the spatial distribution and mass information of molecules, so IMS gets a map of molecule information in the tissue or organ. This label-free method makes a compound analysis more reasonable and prompt than autoradiography, which is a traditional method.

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LC/MS (Liquid chromatography / Mass Spectrometry)

Our company has high-resolution LC-MS equipment and a Triple Quadrupole LC-MS. According to the customer's needs, we provide the best way. Additionally, our team undertakes the service of structural estimation of unknown compounds.

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Salinac Tube

The disposable tube which can be used for LC/MS measurement in phosphate buffer, reduction of Na/K added ions, and reduction of ionization suppression.

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